Core Facilities

Pluripotent Stem Cell Core Facility

A key component of the Stem Cell Engineering Center is the establishment of the stem cell core facility located within the Biotechnology quad on the Georgia Tech campus. The primary emphasis of the core facility is to facilitate stem cell research locally, using human embryonic and induced pluripotent stem cells.

The core facility fosters inter-departmental collaborations among researchers at Georgia Tech by providing samples of stem cells and differentiated cells for preliminary studies, as well as training, technical support and consultation for investigators and laboratories seeking to expand their research programs to work with pluripotent and other stem cells.

Core Activities:

•    Local repository for cell lines & protocols
•    Provide cell samples & lysates for preliminary data
•    Human pluripotent stem cell culture training

The Human Pluripotent Stem Cell core facility is fully-equipped for stem cell culture and the creation of induced pluripotent stem cells. The space is intended to serve as a primary local repository for pluripotent human stem cell lines, as well as cell samples and training for interested investigators.

Equipment:

The core lab houses a standard 4-foot Thermo-Forma biological safety cabinet and a 6-foot custom-modified Thermo-Forma HeraSafe biological safety cabinet which can house an internal stereomicroscope or inverted microscope. A dual stacked 240I copper-lined Heraeus CO2 incubator and a dual stacked 150L copper-lined Heraeus CO2 incubator are used for human pluripotent stem cell culture.

Accuri C6 Flow Cytometer

The Accuri C6 Flow Cytometer has a blue and red laser, four fluorescence and two scatter detectors, event rates of up to 10,000 events/second and sample concentrations over 5x106 cells/mL. The C6 measures the volume pulled from your sample so that cell concentration can be measured without the need for adding beads to samples or performing time-consuming hemocytometer counts.

Nikon Biostation

The BioStation IM is a live cell recorder that allows users to conduct live cell imaging locally or by remote operation over a public or private network. It’s powerful software combined with an integrated monochrome cooled camera captures images of unsurpassed quality. It can be used for cell culture, ADME Tox, Target Identification, molecular pathology, embryology/IVF, cell culture maintenance, cell biology live and regenerative studies. Reserve this equipment.

Bioprofile FLEX Bioanalyzer

The Bioprofile FLEX Bioanalyzer is a cell viability modular instrument that measures up to 16 key cell culture attributes. It is comprised of five separate instruments and can monitor bioreactor runs, determine consumption & production of key metabolites and measure cellular respiration.

Neon Electroporator

The Neon Electroporator has up to 90% efficiency in many cell types, including difficult-to-transfect cells, primary and stem cells. It easily transfects from 2x10^4 cells to 6x10^6 cells per reaction and only needs a single reagent kit for all cell types.

Ambr Micro Bioreactor

Ambr™ mimics the characteristics of classical bioreactors at microscale (10-15ml), by using cost effective, disposable micro-bioreactors, controlled by an automated workstation. The ambr system enables the rapid evaluation of multiple bioreactor cultures at microscale, increasing productivity in cell line development with significant savings on materials and labour. 
View TAP Biosystems Website

The core facility fosters inter-departmental collaborations among researchers at Georgia Tech by providing samples of stem cells and differentiated cells for preliminary studies, as well as training, technical support and consultation for investigators and laboratories seeking to expand their research programs to work with pluripotent and other stem cells.

Location: BME Second Floor
 

Contact: Marissa Cooke

To request cells and/or equipment training, please contact Marissa Cooke.

To reserve equipment, please login to the Human Pluripotent Core Facility on the IBB website.

If you are interested in becoming a faculty member of the Stem Cell Engineering Center, please contact us.  Stem Cell Engineering faculty come from a wide variety of backgrounds including biology, chemistry, physics, biomedical, mechanical, chemical, electrical, and materials science.  
 

The Stem Cell Engineering Center at Georgia Tech is working to innovate novel approaches capable of rapidly accelerating the translation of stem cell science into cellular and molecular therapies, as well as in vitro diagnostic technologies. Learn More.

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