Pluripotent Stem Cell Core Facility
A key component of the Stem Cell Engineering Center is the establishment of the stem cell core facility located within the Biotechnology quad on the Georgia Tech campus. The primary emphasis of the core facility is to facilitate stem cell research locally, using human embryonic and induced pluripotent stem cells.
The core facility fosters inter-departmental collaborations among researchers at Georgia Tech by providing samples of stem cells and differentiated cells for preliminary studies, as well as training, technical support and consultation for investigators and laboratories seeking to expand their research programs to work with pluripotent and other stem cells.
• Local repository for cell lines & protocols
• Provide cell samples & lysates for preliminary data
• Human pluripotent stem cell culture training
The Human Pluripotent Stem Cell core facility is fully-equipped for stem cell culture and the creation of induced pluripotent stem cells. The space is intended to serve as a primary local repository for pluripotent human stem cell lines, as well as cell samples and training for interested investigators.
The core lab houses a standard 4-foot Thermo-Forma biological safety cabinet and a 6-foot custom-modified Thermo-Forma HeraSafe biological safety cabinet which can house an internal stereomicroscope or inverted microscope. A dual stacked 240I copper-lined Heraeus CO2 incubator and a dual stacked 150L copper-lined Heraeus CO2 incubator are used for human pluripotent stem cell culture.
The Accuri C6 Flow Cytometer has a blue and red laser, four fluorescence and two scatter detectors, event rates of up to 10,000 events/second and sample concentrations over 5x106 cells/mL. The C6 measures the volume pulled from your sample so that cell concentration can be measured without the need for adding beads to samples or performing time-consuming hemocytometer counts.
The BioStation IM is a live cell recorder that allows users to conduct live cell imaging locally or by remote operation over a public or private network. It’s powerful software combined with an integrated monochrome cooled camera captures images of unsurpassed quality. It can be used for cell culture, ADME Tox, Target Identification, molecular pathology, embryology/IVF, cell culture maintenance, cell biology live and regenerative studies. Reserve this equipment.
The Bioprofile FLEX Bioanalyzer is a cell viability modular instrument that measures up to 16 key cell culture attributes. It is comprised of five separate instruments and can monitor bioreactor runs, determine consumption & production of key metabolites and measure cellular respiration.
The Neon Electroporator has up to 90% efficiency in many cell types, including difficult-to-transfect cells, primary and stem cells. It easily transfects from 2x10^4 cells to 6x10^6 cells per reaction and only needs a single reagent kit for all cell types.
Ambr™ mimics the characteristics of classical bioreactors at microscale (10-15ml), by using cost effective, disposable micro-bioreactors, controlled by an automated workstation. The ambr system enables the rapid evaluation of multiple bioreactor cultures at microscale, increasing productivity in cell line development with significant savings on materials and labour.
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